Table 5.
Sample dilution. Six samples were analysed undiluted, diluted 1:1 followed by one further dilution 1:1. The diluted sample concentrations were back-calculated as undiluted. For every sample (n =3), calculation of mean, standard deviation, and coefficient of variation for dTG in nmol/L, dC µmol/L, and ratio moles dTG/106 moles dC was done
| Patient | Amount of DNA ng/µL |
dTG nmol/L Mean |
Stdev | %CV | dC µmol/L Mean |
Stdev | %CV | Moles dTG/106 moles dC Mean |
Stdev | %CV |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 27.8 | 1.8 | 0.24 | 12.9 | 1.4 | 0.17 | 9.7 | 1267.5 | 51.4 | 4.1 |
| 2 | 32.1 | 2.3 | 0.17 | 7.5 | 1.9 | 0.09 | 4.6 | 1237.1 | 54.4 | 4.4 |
| 3 | 44.0 | 2.3 | 0.07 | 2.9 | 2.0 | 0.16 | 7.8 | 1148.9 | 57.6 | 5.0 |
| 4 | 34.3 | 1.8 | 0.17 | 9.2 | 2.4 | 0.02 | 1.0 | 748.1 | 75.8 | 10.1 |
| 5 | 66.8 | 0.7 | 0.03 | 4.6 | 1.0 | 0.07 | 6.9 | 763.0 | 73.5 | 9.6 |
| 6 | 61.5 | 1.5 | 0.05 | 3.1 | 0.8 | 0.03 | 3.7 | 1884.3 | 42.9 | 2.3 |
Abbreviations: dTG, 2′-deoxythioguanosine; dC, 2′-deoxycytidin