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. 2024 Oct 13;416(29):6711–6723. doi: 10.1007/s00216-024-05581-6

Table 6.

Comparison between DNA samples, extracted from whole blood at arrival to the laboratory, and stored 4 years at −80°C and DNA samples extracted from frozen whole blood. After 4 years, DNA was extracted from the frozen whole blood and compared with DNA frozen for 4 years. The results from two-sided paired t-test showed no significant difference between dTG, dC, and dTG/dC ratio values between day 0 and 4 years. %RE = (mean−mean day zero)/mean day zero × 100

Day 0 Four years
dTG
nmol/L
dC
µmol/L
Ratio dTG/dC ×10−3 dTG
nmol/L
dC
µmol/L
Ratio dTG/dC
×10−3
Patient
1A 0.09 1.0 0.09 0.10 0.9 0.11
2A 0.56 1.1 0.52 0.50 1.0 0.51
3A 0.38 0.8 0.47 0.39 0.8 0.49
4A 1.26 0.7 1.78 1.47 0.8 1.89
5A 0.73 0.9 0.77 1.13 1.3 0.90
6A 1.69 0.7 2.38 1.88 0.7 2.58
7A 0.93 1.4 0.68 0.77 1.2 0.65
8A 0.56 0.7 0.82 0.54 0.7 0.82
9A 1.24 1.0 1.20 1.20 1.0 1.26
Mean 0.83 0.9 0.97 0.89 0.9 1.02
%RE 7.3 −1.2 5.7

Abbreviations: dTG, 2′-deoxythioguanosine; dC, 2′-deoxycytidine