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. 2024 Nov 20;9:229. doi: 10.1038/s41541-024-01013-9

Fig. 4. Pseudovirus neutralization titers (NT50) elicited by BNT162b2 variant-adapted vaccines administered as a primary series in naive mice.

Fig. 4

Female BALB/c mice (10/group) vaccinated with two-doses of one of the following vaccine regimens at a twenty-one-day interval: monovalent Omicron, BA.4/5, bivalent WT + Omicron BA.4/5, monovalent Omicron XBB.1.5 or bivalent Omicron BA.4/5 + Omicron XBB.1.5. All vaccine formulations contained a total dose of 0.5 µg. Serum neutralizing antibody responses were measured by a pseudovirus neutralization assay at one-month post-second dose against the WT reference strain, the Omicron sublineages BA.4/5, XBB.1.5, XBB.1.16, XBB.1.16.1, XBB.2.3, EG.5.1 and the lineage BA.2.86. A 50% pseudovirus neutralization titers are shown as geometric mean titers (GMT) ± 95% CI of 10 mice per vaccine group. Each point represents an individual animal. B The geometric mean ratio (GMR) is the GMT of individual pseudovirus responses of each vaccine group (monovalent Omicron BA.4/5, monovalent Omicron XBB.1.5 or bivalent Omicron BA.4/5 + Omicron XBB.1.5) divided by GMTs of analogous pseudovirus responses of the bivalent WT + Omicron BA.4/5 group. Statistical differences were analysed by ANOVA with a Dunnett’s multiple comparison test (**p < 0.01; ****p < 0.0001). The limit of detection (LOD) is the lowest serum dilution, 1:20.