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. 2024 Nov 20;7:1540. doi: 10.1038/s42003-024-07196-0

Fig. 3. Effects of BK treatment on cell senescence and extracellular matrix protein expression in nucleus pulposus cells.

Fig. 3

A Expression of cell senescence staining after TBHP and BK treatment at 100x and 1000x magnification. B Quantitative statistics of positive cells after senescence staining. C Western blot analysis of P16, P21 and P-P53 in cells treated with BK and TBHP. D–F Quantitative analysis of P16, P21 and P-P53 expression levels. G Aggrecan immunofluorescence staining of cells after TBHP and BK treatment at 200x magnification. H Quantitative statistics of the average immunofluorescence intensity of cells in each experimental group. I Western blot analysis of Aggrecan and Collagen-II in cells treated with BK and TBHP. J, K Quantitative analysis of Aggrecan and Collagen-II expression levels. L MMP9 immunofluorescence staining of cells after TBHP and BK treatment at 200x magnification. M Quantitative statistics of the average immunofluorescence intensity of cells in each experimental group. N Western blot analysis of MMP-3 and MMP-9 in cells treated with BK and TBHP. O, P Quantitative analysis of Aggrecan and Collagen-II expression levels. Q Western blot analysis of PI3K, P-PI3K, AKT and P-AKT in cells treated with BK and TBHP. R–U Quantitative analysis of P-PI3K/ACTIN, P-AKT/ACTIN, P-PI3K/PI3K, and P-AKT/AKT ratios. To assess the statistical differences between various groups, we employed one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test for multiple comparisons (*: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001). There are no significant statistical differences in Fig. 3F, K, O, and R.