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. 2024 Nov 20;7:1540. doi: 10.1038/s42003-024-07196-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 4 — A, B SA-β-gal senescence staining and quantitative analysis of senescent cell counts. HOE140 and LY294002 can block the anti-aging effect of BK. C–F Western blot analysis and quantitative statistical analysis of the expression of senescence markers P-P53, P21 and P16 in NPCs. G, H Aggrecan immunofluorescence and quantitative analysis of fluorescence intensity of cells. HOE140 and LY294002 can block the anabolic-promoting effect of BK. I–K Western blot analysis and quantitative statistical analysis of the expression of extracellular matrix anabolic markers Aggrecan and Collagen-II in NPCs. L, M MMP-9 immunofluorescence and quantitative analysis of fluorescence intensity of cells. HOE140 and LY294002 can block the effect of BK on reducing extracellular matrix catabolism of NPCs. N–P Western blot analysis and quantitative statistical analysis of the expression of extracellular matrix catabolism markers MMP-3 and MMP-9 in NPCs. Q Western blot analysis of the expression of PI3K, p-PI3K, AKT and pAKT in NPCs. R–U Quantitative analysis of P-PI3K/ACTIN, P-AKT/AKT, P-PI3K/PI3K and P-AKT/ACTIN levels.