Fig. 2. Details of CDH1^α1 interaction with APC1^WD40.

a Overview of CDH1^α1:APC1^WD40 interactions. b Detailed view of CDH1^α1 interacting with APC1^WD40. c Cryo-EM density map showing CDH1^α1. d An equivalent, although shorter N-terminal α-helix exists in CDC20. Map of CDC20^α1:APC1^WD40 interactions (from EMDB: 4466). CDC20^α1 is based on the AlphaFold model of human CDC20²⁹. e Superimposition of APC/C^CDH1:EMI1 onto apo-APC/C showing how CDH1^α1 overlaps with the C-terminal region of the APC1^300s loop (labelled as APC1⁴⁰²) of apo-APC/C (coloured light grey). Multiple sequence alignments showing conservation of metazoan CDH1^α1 (f) and CDC20^α1 (g).