Figure 3. Expression of the mouse BA carriers mNtcp and mAsbt in HEK293 cells. A. Immunofluorescence microscopy of mAsbt-HEK and mNtcp-HEK cells. The images show anti-FLAG-fluorescence (green fluorescence). Nuclei were stained with Hoechst 33258 (blue fluorescence). HEK293 cells expressing the human Ntcp-FLAG protein were used as positive control and immunostaining without the anti-FLAG primary antibody was used as negative control. B. Expression of functionally active mNtcp and mAsbt carriers was verified by sodium-dependent uptake of 3β-NBD-TCA.
