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. 2024 Nov 4;9(46):46362–46372. doi: 10.1021/acsomega.4c07398

Figure 4.

Figure 4

Evaluation of reliable identification results of HFDiscrim on the MCB data set. (A–C) Identification consistency of mouse precursors, peptides, and proteins at 1% precursor FDR using the external method. Only precursors identified in all 10 runs on the MCB data set were considered. (D) Logarithmic intensity distributions of the mouse precursors identified at 1% precursor FDR using the external method. All precursor identification records in all 10 runs were considered. Logarithmic intensities were z-normalized by subtracting the mean intensity and then dividing by the standard deviation to make the distributions comparable. (E) Natural logarithm (ln) intensities of the precursors uniquely identified by HFDiscrim but not by PyProphet, in all 10 biologically independent runs on the MCB data sets.