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. 2024 Nov 20;46(2):2431150. doi: 10.1080/0886022X.2024.2431150

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© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 3. — Effects of ERK phosphorylation agonist Ro 67-7476 on viability, apoptosis, migration, and LONP1 expression in podocytes co-treated with PAN and shTRPC6.

AB8/13 cells were transfected with shTRPC6, and then treated with 30 µg/ml PAN for 48 h and Ro 67-7476 for 1 h. (A) The viability of AB8/13 cells in the Control, PAN, PAN+shTRPC6, and PAN+shTRPC6 + Ro 67-7476 groups was detected by cell counting kit-8 assay. (B, D) The apoptosis rate of AB8/13 cells in each group was evaluated by flow cytometry. (C, E) The migration rate of AB8/13 cells in each group was determined by transwell assay (magnification: ×250, scale bar = 50 µm). (F-G) LONP1 protein expression in each group was quantified by Western blot. GAPDH was taken as the loading control. The data are presented as the mean ± standard deviation of three independent experiments; ***p < 0.001 vs. Control; ⁺⁺p < 0.01, ⁺⁺⁺p < 0.001 vs. PAN; ^△△p < 0.01, ^△△△p < 0.001 vs. PAN+shTRPC6. Abbreviation: TRPC6, transient receptor potential cation channel subfamily C member 6; shTRPC6, short hairpin RNA targeting TRPC6; PAN, puromycin aminonucleoside; LONP1, Lon peptidase 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.