TABLE 1.

Primers and probes used for the detection of Hepatovirus A (HAV) nucleic acids, published, and validated by Jothikumar et al. (24)^a

Primer or probe	Sequence
Forward	GGTAGGCTACGGGTGAAAC
Reverse	AACAACTCACCAATATCCGC
Probe	CTTAGGCTAATACTTCTATGAAGAGATGC

^a Primers and probes were purchased from Integrated DNA Technologies (Coralville, IA, USA). The probes contained fluorescent molecule FAM and quenchers (5′ FAM/ZEN/3′ IBFQ); FAM, fluorescein amidite; ZEN, a proprietary internal quencher from Integrated DNA Technologies (Coralville, IA, USA); and IBFQ, Iowa Black FQ. Amplicon size is 89 base pairs.