1987–1989	Toxin-coregulated pilus (TCP) is the major virulence factor (36). TnphoA-mediated discovery of TCP pilin and other exported virulence factors (22, 37). Defined the ToxR binding site within the cholera toxin operon promoter (23, 38).
1990	Anti-TCP antibodies protect against infection (39).
1992	TcpG catalyzes disulfide bond formation in multiple virulence proteins (40).
1995–2000	Identified key residues on TCP for microcolony formation, adherence, and antigenicity (41–43).
1997–2000	cAMP-CRP and H-NS repress virulence genes outside the host (44, 45). Identification of environmental signals controlling virulence gene regulation (35).
2003–2011	TcpF is a TCP-dependent secreted factor that is essential for colonization (46–49). GbpA binds chitin in the environment and receptors in the small intestine (50).