TABLE 4.
Quantitative PCR reagent sequences
| Gene target | Reagent | Sequence 5′−3′ | Origin |
|---|---|---|---|
| C. coli cadF | Forward primer | GAGAAATTTTATTTTTATGGTTTAGCTGGT | (58) |
| Reverse primer | ACCTGCTCCATAATGGCCAA | ||
| Probe | 6FAM]CCTCCACTTTTATTATCAAAAGCGCCTTTAGAA[BHQ2] | ||
| C. coli ceuE a | Forward primer | AAGCTCTTATTGTTCTAACCAATTCTAACA | (59) |
| Reverse primer | TCATCCACAGCATTGATTCCTAA | ||
| Probe | [6FAM]TTGGACCTCAATCTCGCTTTGGAATCATT[BHQ2] | ||
| C. coli glyA a | Forward primer | AAACCAAAGCTTATCGTGTGC | (60) |
| Reverse primer | AGTGCAGCAATGTGTGCAATG | ||
| Probe | [6FAM]CAACTTCATCCGCAAT[BHQ2] | ||
| C. jejuni gyrA | Forward primer | AAGATACGGTCGATTTTGTTCCA | (61) |
| Reverse primer | CTACAGCTATACCACTTGAACCATTTAATA | ||
| Probe | [FAM]TGATGGTTCAGAAAGCGAACCTGATGTTTT[BHQ2] | ||
| NHP OSM | Forward primer | CCTCGGGCTCAGGAACAAC | (62) |
| Reverse primer | GGCCTTCGTGGGCTCAG | ||
| Probe | [TAM]TACTGCATGGCCCAGCTGCTGGACAA[BHQ2] |
a
These primer/probe combinations performed poorly compared to the cadF assay and were not used in sample testing.