FIGURE 6.

Treatment with bFKB1 reduces formation of new collagen in the liver and reduces hepatic expression of upstream regulators of fibrosis. Representative histological photomicrographs of Sirius Red‐stained liver cross‐sections (A), fibrosis as percentage of surface area (B), fibrosis stage (C), and absolute newly formed hydroxyproline (D). Values are presented as mean ± SEM for n = 15 mice on HFD treated with isotype control antibody and n = 13 mice on HFD treated with bFKB1. **p < .01 bFKB1 versus isotype control antibody. Activity of upstream regulators in homogenized liver tissue is expressed as z‐score that indicates the predicted activation state of a transcription factor or regulator. A z‐score <−2 indicates relevant inhibition (green) and z‐score >2 indicates relevant activation (red). The p‐value indicates significant enrichment of genes downstream of a regulator with p < .01 was considered statistically significant (gray). Hepatic expression of genes involved in transforming growth factor (TGF)‐beta signaling and other regulators of fibrosis (E).