Figure 1.

Overview of the yeast one‐hybrid (Y1H) system. (A) The bait construct is obtained by cloning a DNA sequence of interest (Bait DNA sequence) upstream of a reporter gene (HIS3) carrying a core promoter. The prey construct is obtained by cloning a protein of interest in frame with the GAL4 activation domain (GAL4‐AD). After mating or co‐transformation, although negative control prey(s) should not bind the bait DNA sequence, there might be a basal expression of the reporter gene HIS3 that can be titrated by the competitive inhibitor 3‐AT. (B) If the prey binds to the bait DNA, transcription of the reporter gene HIS3 will be activated, overcoming the titration with 3‐AT and allowing yeast growth.