Figure 6.
GH injection increases the expression of c-Fos in LHAORX neurons. A–E, The percentage of ORX neurons expressing c-Fos and representative photomicrographs of C57BL/6J mice that received an acute injection of saline (A, B; n = 5) or GH (C, D; n = 6). c-Fos protein is observed through black nuclear staining, whereas ORX neurons present cytoplasmic brownish staining. Arrows indicate double-labeled neurons. B and D represent higher-magnification photomicrographs of the selected areas in A and C, respectively. Abbreviation: fx, fornix. Scale bar, 100 µm. Differences between groups were analyzed by unpaired two-tailed Student’s t test. *p < 0.05 significant difference between groups.