Figure 3.

Histological observations of male zebrafish testes after 2-month in vivo exposure to DBDPE. (A−E) Typical hematoxylin/eosin–stained sections of testes in 0, 0.1, 1, 10, and $100\text{\hspace{0.17em}nM}$-exposure groups, respectively. (F) Proportion quantification of germ cells at different developmental stages in testes ($n=6–9$ fish/group). Results are represented as $\text{means}\pm \text{standard}$ errors of the mean (SEMs). Scale bar: $50\mathrm{\mu }\mathrm{m}$. Data are reported in Excel Table S3. Note: DBDPE, decabromodiphenyl ethane; SPC, spermatocytes; SPD, spermatozoa; SPG, spermatogonia. *$p<0.05$, **$p<0.01$, and ***$p<0.001$ indicate significant differences between exposure and control groups, by one-way analysis of variance (ANOVA) followed by the post hoc least significant difference (LSD) test.