Fig. 3. Isothermal assembly of DNA nanostructures in calcium.

(a) Scheme and models of the DNA nanostructures used in this study. (b-e) Isothermal assembly at different temperatures (shown by the gradient) in buffer containing different Ca²⁺ concentrations for the 4-arm junction, DX-E, 3-helix motif, and 4-helix motif, respectively. (f) Melting curves of structures annealed and isothermally assembled in Ca²⁺. Data represent mean and error propagated from standard deviations of experiments performed in triplicates.