Fig. 4. Rationally designed 3D DNA crystals assembled in calcium.

(a) Scheme of the tensegrity triangle motif containing three double-helical edges connected by strand crossovers. (b) Model of the tensegrity triangle motif with 3 turns per edge and assembly into a crystal lattice. The triangle is 3-fold symmetric and contains the same sequence on each edge. The edges of the triangle are shown in different shades in the illustration for clarity. (c) Growth of 3D DNA crystals from motifs annealed or isothermally assembled in buffer containing calcium.