Fig. 6. Isothermal assembly in different ions.

(a-e) Isothermal assembly of DX-O motif in different ions at 4 °C, 20 °C, 37 °C and 50 °C. (f) Isothermal assembly of DX-O motif in Ni⁺ at 4 °C, 20 °C, 37 °C and 50 °C. (g) Assembly of DX-O in 10 and 100 mM Ni²⁺ incubated for 2 h at different temperatures from 30 to 50 °C. (h) Effect of Ni²⁺ and temperature on DX-O motif isothermally assembled in Ni²⁺ or annealed in Mg²⁺. Data represent mean and error propagated from standard deviations of experiments performed in triplicates.