Figure 4. SET-32 and SET-21 are required for H3K23me3 and H3K9me3 at germline nuclear RNAi targets.

(A) Venn diagram of numbers of regions with HRDE-1 and SET-32/21-dependent H3K23me3 (H3K23me3 [mutant/WT]≤2/3, FDR≤0.05). (B) Box plot of H3K23me3 levels (relative to WT) for regions with SET-32/21-dependent H3K23me3 in different mutant strains. Mann-Whitney U tests (two-sided) were used to determine the statistical significance for the H3K23me3 differences between a mutant and WT (null hypothesis: no difference). (C-D) Venn diagram of numbers of regions of H3K23me3 and H3K9me3 that are dependent on (C) HRDE-1, (D) SET-32 and SET-21, and (E) MET-2 and SET-25 (H3K23me3 or H3K9me3 [mutant/WT]≤2/3, FDR≤0.05). Hypergeometric distribution was used to calculate the p-values of the significance of the overlaps in the Venn diagrams (null hypothesis: no significant overlap).