Figure 8. The requirement of SET-32 and SET-21 for (A,B) RNAi, (C,D) piRNAi, and (E) cosuppression.

(A and C) The schematics of heritable RNAi by dsRNA feeding (A) and heritable piRNAi (C), both against oma-1. (B) oma-1 pre-mRNA levels measured by RT-qPCR for heritable RNAi in WT and various mutants. (D) oma-1 mRNA levels measured by RT-qPCR for heritable piRNAi. The values were normalized to the tubulin gene tba-1 mRNA expression from the same sample and relative to the control samples. (E) mRNA levels of the native oma-1 gene, measured by RNA-seq, for the cosuppression experiment in strains carrying the oma-1 transgene. The values were normalized to a germline expressed gene pie-1 mRNA levels.