Figure 4. Depletion of both Numb and Mad leads to more EB/EB division.

(A) Scheme of an ISC division that produces differentially labeled daughter cells (RFP⁺ GFP⁻ and RFP⁻ GFP⁺) through FRT-mediated mitotic recombination. Adapted from (Tian and Jiang 2014).

(B) Scheme of differentially labeled twin clones generated by FLP/FRT-mediated mitotic recombination of dividing ISCs. Adapted from (Tian and Jiang 2014).

(C) Scheme of twin-spot experiments. 3~5-day-old adult flies of indicated genotype are grown at 29°C for 14 days before heat shock to induce clones. After one-day recovery at 29°C, the flies are raised at 18°C for 4 days prior to analysis.

(D-O) Representative images of twin-spot clones from adult midguts of the indicated genotypes. Scale bar 20 μm is shown in (D).

(P) Quantification of twin spots of different classes from guts of the indicated genotypes. Data are mean ± SD from three independent experiments. *, p < 0.05, **, p < 0.01.