Figure 6. Numb is critical for ISC maintenance during regeneration.

(A-I’) Adult flies of indicated genotype were treated with sucrose, bleomycin or DSS for 24h at 14 days after clone induction and recovered for another 4 days before dissection. Guts containing MARCM clones of the indicated genotype were stained for GFP (green) and Dl (red and white). GFP marks the clones. DAPI (blue) staining indicates the nuclei. Stem cells inside and outside the clones are indicated by yellow and white arrows, respectively. Scale bar (20 μm) is shown in (A).

(J) Quantification of the percentage of clones with or without ISCs.

(K) Quantification of clone size distribution for the indicated genotypes.

Data are mean ± SD from three independent experiments.*, p < 0.05, **, p < 0.01 ****, p < 0.0001.