Figure 5. Rad52 promotes Rad51 binding to ssDNA.

A) Schematic of the optical trap experiment showing Rad51^GFP binding to ssDNA in the absence/presence of Rad52. Kymographs of optical trap data in the B) absence or C) presence of Rad52 shows stimulation of Rad51^GFP binding to ssDNA. D) Kymograph showing no binding of Rad51^GFP to an RPA-coated ssDNA in the absence of Rad52. E) Rad52^ΔC that lacks the C-terminal Rad51 and RPA interaction domains poorly promotes Rad51^GFP binding to ssDNA. F) Poor binding of Rad51^GFP binding to dsDNA is observed. G) Quantitation of Rad51^GFP filaments formed under the denoted conditions. Representative data from a minimum of n=5 tethers per condition is shown, and Std. Dev. of the mean is plotted.