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[Preprint]. 2024 Nov 8:2024.11.08.620918. [Version 1] doi: 10.1101/2024.11.08.620918

Figure 1. Single-cell RNA sequencing data analysis reveals expression of classical ionocyte-marker genes in the zebrafish olfactory organ.

Figure 1.

(A) UMAP plot showing 24 unannotated cell clusters (Identity) in the scRNA-seq dataset generated from dissected adult zebrafish olfactory organs. (B) Dot plot of data from B depicting several known ionocyte markers expressed in cluster 18. (C) Feature plots for foxi3b, (D) foxi3a, (E) ceacam1 and (F) trpv6. (G) Maximum intensity projection of a 4 dpf larva stained with DAPI, frontal view shows the two olfactory pits. Scale bar: 50 μm. (H) UMAP plot of taste/olfactory subset from Daniocell [34]. (I) Zoomed in feature plots for the ionocyte markers foxi3b, foxi3a, ceacam1, trpv6 and gcm2. (J) UMAP plot of olfactory subset from [17]. (K) Dot plot of data from J showing several NCC ionocyte markers in foxi3b+ cells in the olfactory epithelium. (L) Feature plots for foxi3b and (M) slc12a10.2.