Figure 5. Notch signalling is required for paired ionocyte survival, but does not affect NCC-like ionocyte number.

(A) Schematic of experimental design. (B) Number of NaR- and HR-like ionocyte pairs in DMSO controls and treated with the Notch inhibitor LY411575. Mann Whitney test (P < 0.0001). (C) Number of NCC-like ionocytes in DMSO controls and treated with the Notch inhibitor LY411575. Unpaired t-test (P = 0.7070). (D–D’’) Representative maximum intensity projection confocal images of HCR RNA-FISH for trpv6 (yellow) and foxi3b (cyan) with DAPI stain (grey) in DMSO-treated and (E–E’’) LY411575-treated olfactory pits. N = 14 olfactory pits per condition. Scale bars: 20 μm.