Fig 4. Quantification of the degradation of the IVT-mRNA product.

A) Representative profile of ladder using lane background subtraction with manufacturer-defined molecular weight. B) Show the best-fit exponential curve and formula for molecular weight assessment based on the position of the gel. C) Decreases in maximal values of molecular weight of mRNA after various times of incubation at 37 degrees. D) Peak shift defined by the difference of maximal molecular weight values after incubations. E) Quantification of maximal values differences. *, p<0.05, **, p<0.01, ***, p<0.001 determined by ANOVA followed by Tukey test (N = 3).