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. 2024 Nov 11;20(11):e1012312. doi: 10.1371/journal.ppat.1012312

Fig 5. Proximity-labeling proteomic analysis suggests TRIM32 associates with the endosome-lysosome compartment.

Fig 5

A. Schematic of proximity labeling proteomic approach for identification of cellular proteins and viral proteins of VEEV that interact with TRIM32. B. HeLa-TurboID-HA or HeLa-TRIM32-TurboID-HA cells were infected with VEEV-TC83-GFP at 0.1 MOI for 12h, virus infectivity was quantified by flow cytometry. C. The protein abundance of TRIM32 and viral structural and nonstructural polyproteins from the indicated groups. Triangle, HeLa-TurboID-HA infected with (blue) or without (red) infection. Circle, HeLa-TRIM32-TurboID-HA infected with (blue) or without (red) infection. D-E. Confocal images of the intracellular localization of TRIM32 and the indicated endosome or lysosome markers. Data represent averages of independent biological replicates and are presented as means ± SD (n = 2–3). Statistical significance was determined by unpaired students’ t-test for A and C (****P<0.0001).