Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HMGU1; Human induced pluripotent stem cells | kind gift of Dr. Drukker |
HMGU1; source cells: BJ (ATCC CRL-2522) | MTA approval was obtained from the Helmholtz Zentrum München (HMGU), Germany |
| Peptide, recombinant protein | Recombinant Human/Mouse FGF-8b Protein | R&D | 423-F8-025/CF | (100 ng/ML) |
| Peptide, recombinant protein | BDNF | PeproTech | #450–02 | 10 ng/mL |
| Commercial assay or kit | hPSC genetic Analysis kit | Stem Cell Technologies | #07550 | hiPSCs culture |
| Commercial assay or kit | NucleoSpin RNA II columns | Macherey-Nagel | 740902.50 | hiPSCs culture |
| Commercial assay or kit | Reverse Transcriptase Core Kit | Eurogentec | RT-RTCK-03 | hiPSCs culture |
| Chemical compound, drug | Matrigel | Corning | 354234 | 5–10 µl matrigel dissolved in 1 ml cold DMEM-F12 for each well of a 6-well culture plate |
| Chemical compound, drug | mTeSR1 medium | STEMCELL Technologies | #85850 | hiPSCs culture |
| Chemical compound, drug | ROCK inhibitor Y-27632 | MedChemExpress; or Stem Cell Technologies | MedChemExpress HY-10583; or Stem Cell Technologies #72304 | 10 µM |
| Chemical compound, drug | DMEM/F12 | Thermo Fisher Scientific | #31331028 | hiPSCs culture |
| Chemical compound, drug | N-2 Supplement 100X | Thermo Fisher Scientific | 17502–048 | hiPSCs culture |
| Chemical compound, drug | B-27 Supplement 50X, minus vitamin A | Thermo Fisher Scientific | 12587–010 | hiPSCs culture |
| Chemical compound, drug | LDN-193189 | Sigma | SML0559-5MG | 0.25 µM |
| Chemical compound, drug | SB-431542 | Sigma | S4317-5MG | 5 µM |
| Chemical compound, drug | XAV-939 | Stem cell technologies | 72674 | 2 µM |
| Antibody | CTIP2, FOXG1, GAD1, OTX2, MAP2, ASCL1, NR2F1, NTUB (ACETILATED TUBULIN), OCT-3/4, PAX6, SATB2, SOX2, TBR1, TUJ1, SLC17A6, SLC17A7 antibodies | See Table 2 for supplier information and antibody host species | See Table 2 for reference codes | See Table 2 for antibody dilution |
| Sequence-based reagent | DUSP6, EN2, ETV1, ETV4, ETV5, FOXG1, GAPDH, NANOG, NR2F1, OCT4, OTX2, PAX6, SIX3, SOX2, SPRY4, B-ACTIN primers | This paper | PCR primers | See Table 1 for sequence of forward and reverse primers |