FIGURE 2.

Nicotinamide mononucleotide (NMN) treatment restores signal transducer and activator of transcription 1 (STAT1) level in the colitis‐associated cancer (CAC) tissues. (A) Experimental variations of colon proteomics analysis between the control and NMN‐treated groups under azoxymethane/dextran sulfate sodium (AOM/DSS) condition. (B) Volcano plot representing differences in proteins between the control and NMN‐treated groups. (C) Protein–protein interactions of differentially expressed proteins (DEPs) constructed using Cytoscape (V3.10.0). Red and blue fonts indicate upregulated and downregulated proteins, respectively. Yellow nodes are proteins that interact with STAT1. (D) Gene ontology (GO) annotation of DEPs. (E) Western blotting verification of STAT1 and phosphorylated STAT1. Means ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; one‐way analysis of variance (ANOVA) test.