Fig. 6.
No influence of specific S1P receptor agonists on PMCA1 expression. A mRNA expression of S1PR1-S1PR5 in control cells (shown as 2ΔCt) and SphK1-KD2 cells (shown as 2ΔΔCt, relative to control cells). B, C Protein expression of PMCA1 after treating control or SphK1-KD2 cells with vehicle, CYM5442 (1 µM), CYM5541 (1 µM), CYM50308 (1 µM), CYM5520 (5 µM), or A971432 (1 µM) for 16 h, respectively. Shown are values from three independent experiments (means ± SEM; *p < 0.05; **p < 0.01 in one sample t-test)