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. 2024 Nov 13;31(1):29. doi: 10.3892/mmr.2024.13393

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Copyright: © 2024 He et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Isolation and identification of DPSC and DPSC-EVs. (A) DPSCs were isolated, cultured and observed under a microscope. (B) Flow cytometry was performed to detect DPSC markers (CD44, CD90, CD34 and CD45). (C) ALP staining, Alizarin red staining and Oil Red O staining were performed to evaluate the osteogenic capacity of DPSCs. (D) The morphological features of DPSC-EVs were observed. The average size of EVs was 115 nm. (E) The NTA particle size of DPSC-EVs was analyzed. (F) Western blotting was performed to detect DPSC-EV markers (CD9, CD63, CD81, TSG101 and calnexin). Triplicate independent experiments were used. DPSC, dental pulp stem cell; EV, extracellular vesicle; NTA, nanoparticles tracking analysis; FL1, green fluorescent channel; P1,first generation DPSCs cells; P3, third generation DPSCs cells; TSG101,tumor susceptibility gene 101; TEM, transmission electron microscopy.