Fig. 5. Effect of cytoskeleton disruption on cell deformability and opacity.

Density plots of electrical deformability vs electrical diameter for (a) untreated, (b) 1 μM CytoD and (c) 0.25 μM LatB treated cells. Contour plots (50% density) of (d) electrical deformability (d) and electrical opacity vs electrical diameter (e) for CytoD-treated cells. Dose-response graph for electrical diameter (f), electrical deformability (g) and opacity (h). CytoD deformability EC₅₀ for = 17 nM. Error bars are standard errors of the mean value of n = 6. P-values (Student’s t-test) are relative to untreated cells. All data collected at a flow rate of 5 μl/min