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. 2024 Nov 21;14:28846. doi: 10.1038/s41598-024-79854-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 1 — Acquired images (20X objective). The optical microscopy H&E (A-D), second harmonic generation microscopy (E-H), two-photon excited fluorescence microscopy (I-L) and the extracted collagen fibres with each fibre in a random colour (M-P) for the human normal mammary tissue (A, E, I and M), fibroadenoma (B, F, J and N), grade I invasive carcinoma (C, G, K and O) and invasive micropapillary carcinoma (D, H, L and P). Asterisk HE: stroma. HE arrowhead: epithelial cells. The scale bar is 100 μm.