Figure 3.

Projection view of the 2DSA-Monte Carlo sedimentation profile as a function of wavelength for the 50:50 DNA–BSA mixture. Remarkably, the protein absorbance spectrum at 4.3 S (two yellow peaks) can be clearly distinguished from the adjacent DNA peak with absorbance maximum around 258 nm, despite the proximity of the peaks (4.5 S vs. 5.2 S). Minor species can be identified based on their spectra. The straight lines attest to the high resolution and robustness of this approach to fit multiwavelength data (each wavelength is separately analyzed).