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. Author manuscript; available in PMC: 2024 Nov 22.
Published in final edited form as: Methods Enzymol. 2015 Aug 21;562:27–47. doi: 10.1016/bs.mie.2015.04.013

Figure 6.

Figure 6

van Holde–Weischet differential distribution projection plot as a function of wavelength for the BSA–DNA 50:50 mixture. The asymmetric shape of the peak between 4 and 6 S clearly indicates a heterogeneity in spectral properties originating from the closely sedimenting BSA and 208 bp DNA peaks. On the left side of the peak, the BSA extinction dominates;on the right side of the peak, the DNA extinction profile dominates. The peak at 11 S displays a DNA extinction profile only. The color (different gray shades for the print version) gradient indicates relative concentration.