Skip to main content
. Author manuscript; available in PMC: 2024 Nov 22.
Published in final edited form as: Methods Enzymol. 2015 Aug 21;562:27–47. doi: 10.1016/bs.mie.2015.04.013

Figure 7.

Figure 7

Global genetic algorithm Monte Carlo analysis of decomposition results obtained from six different DNA and BSA mixtures analyzed on the open AUC MWL instrument (top left) and the dual-wavelength results obtained from the Beckman-Coulter XL-A (right panel). The separate decomposition results for DNA (red) and BSA (blue) are combined in the left panel pseudo-3D plot to illustrate the exceptional separation achieved by spectral decomposition which even separates species with nearly identical sedimentation coefficients (the two major species sedimenting near 5 S). This approach demonstrates the superior resolution obtained from MWL analysis compared to the global two-wavelength analysis performed on the Beckman-Coulter XL-A (right panel, green). Lower panel: differential distributions from the same data shown above (red): decomposition for DNA, blue: decomposition for BSA, green: nonseparated XL-A data for DNA-BSA mixtures globally fitted to genetic algorithm–Monte Carlo analysis. A color representation of this figure is provided in the color plate section to allow the reader to distinguish the DNA and BSA contributions based on color.