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. Author manuscript; available in PMC: 2024 Nov 22.
Published in final edited form as: Methods Enzymol. 2015 Aug 21;562:27–47. doi: 10.1016/bs.mie.2015.04.013

Figure 8.

Figure 8

Comparison of results from the dual-wavelength AUC/GA analysis with the spectral decomposition method to ascertain the relative composition of spectrally different components in mixtures. The results clearly show that the spectral decomposition achieves even higher fidelity in reproducing the known target values than single-wavelength AUC/GA. Samples 1–5 refer to ratios of 20%, 35%, 50%, 65%, and 80% BSA in a BSA:DNA mixture with average errors of around 0.7%. Errors observed from the decomposition MWLD analysis results are on par with the spectral decompositions of the Genesys spectrophotometer data and are at least as good as the data obtained from the Beckman-Coulter XL-A.