Figure 1.

Enzyme-responsive DNA condensates. a) Schematic representations exemplifying the endonuclease and glycosylase activity that can be localized in DNA condensates. b) Schematic representation of enzyme-mediated dynamic patterning of DNA condensates. When added, an enzyme digests nucleic acid substrates bound to the condensates. Digested substrates can be replaced by fresh strands present in solution, producing reaction-diffusion patterns dependent on enzymatic activity, the diffusivity and binding strength of multiple, coexisting substrates. c) Schematic representation of DNA condensates with two concentric subcompartments each containing a different enzymatic substrate, allowing enzymatic reactions to occur only within the predetermined subcompartments.