Fig. 1.

Activation of MST1 is involved in the pathological process of 5xFAD mice. A Representative thioflavin S staining images for detection content in the hippocampus of 1-, 3-, 6-, 9-month-old WT and 5xFAD mice. B Quantitative analysis of the fluorescence intensity of amyloid plaque in the hippocampus of 1-, 3-, 6-, 9-month-old WT and 5xFAD mice (n = 3). C Hippocampus MST1, p-MST1, Bax, and Bcl-2 protein levels in 3-, 6-, 9-month-old WT and 5xFAD mice as showed using immunoblotting analysis. D Quantitative analysis of p-MST1/MST1 (n = 4). E Quantitative analysis of Bax/Bcl-2 (n = 4). F–I Representative immunohistochemical images and relative expression of p-MST1 in the hippocampus (F, G) and cortex (H, I) of 6-month-old WT and 5xFAD mice (n = 3, Scale bar is 200 µm and enlarged images scale bar is 20 µm). Nuclei were stained in blue (DAPI). All data represent means ± SEMs. *, 5xFAD vs. WT group at different months of age. *p < 0.05; **p < 0.01 and ***p < 0.001. #, Comparison between 5xFAD at different months of age, #p < 0.05; ##p < 0.01