Fig. 7.
MST1 effects upon cell viability and mitochondria in vitro. A, B Western blotting measured the expression of MST1 and p-MST1 in SH-SY5Y cells cultured in 0, 5, 10, 20 and 40 μM Aβ1-42 for 24 h. B Quantitative analysis of p-MST1/ MST1 (n = 3). C, D Representative immunofluorescence images (C) and quantitative analysis (D) of p-MST1 in control and Aβ-treated groups (Scale bar is 20 μm). Nuclei were stained in blue (DAPI). (n = 3). E CCK8 assay was used to detect the cell viability of SH-SY5Y cells after treatment with Aβ1-42 or MST1 overexpressed plasmid. (n = 3) F CCK8 assay was used to detect the cell viability of SH-SY5Y cells after treatment with Aβ1-42 or MST1 specific siRNA (n = 3). G, H Flow cytometry analysis cells apoptosis of SH-SY5Y cells in different groups (Control, Aβ, ad-MST1 + Aβ, si-MST1 + Aβ). I Representative images of TMRM staining in SH-SY5Y cells in the Control, Aβ, ad-MST1 + Aβ, si-MST1 + Aβ groups (Scale bar is 20 μm). Nuclei were stained in blue (DAPI). J Quantitative analysis of TMRM. (n = 3). K Representative images of mitochondrial ROS in SH-SY5Y cells in the Control, Aβ, ad-MST1 + Aβ, si-MST1 + Aβ groups measured by MitoSOX Red staining (Scale bar is 20 μm). Nuclei were stained in blue (DAPI). L Quantitative analysis of MitoSOX Red staining (n = 3). All data represent means ± SEMs. *, Compared with the control group, *p < 0.05, **p < 0.01 and ***p < 0.001. #, Comparison between intervention groups, #p < 0.05; ##p < 0.01