Fig. 9.

MST1 affects oxidative stress through PI3K-Akt pathway, thereby affecting mitochondrial function. SH-SY5Y cells were incubated with 25 μM of PI3K-Akt activator (740 Y-P) for 1 h before treatment with Aβ after transfection with MST1 overexpression plasmid. SH-SY5Y cells were incubated with 20 μM of PI3K-Akt Inhibitor (LY294002) for 1 h before treatment with Aβ after transfection with MST1 specific siRNA. A, B Relative levels of PI3K, Akt, p-Akt, Bax, Bcl-2, Cleaved-Caspase 3, and Cyt-C protein in control, Aβ, ad-MST1 + Aβ, ad-MST1 + Aβ + 740Y-P group (n = 3). C, D Relative levels of PI3K, Akt, p-Akt, Bax, Bcl-2, Cleaved-Caspase 3, and Cyt-C protein in control, Aβ, si-MST1 + Aβ, si-MST1 + Aβ + LY294002 group (n = 3). E–H Relative intensity of ROS by Flow cytometry analysis in different groups. I, J Representative images (I) and quantitative analysis (J) of TMRM staining in SH-SY5Y cells in the Control, Aβ, ad-MST1 + Aβ, ad-MST1 + Aβ + 740Y-P groups (Scale bar is 20 µm). Nuclei were stained in blue (DAPI). (n = 3). K, L Representative images (K) and quantitative analysis (L) of TMRM staining in SH-SY5Y cells in the Control, Aβ, ad-MST1 + Aβ, si-MST1 + Aβ + LY294002 groups (Scale bar is 20 µm). Nuclei were stained in blue (DAPI). (n = 3). All data are repeated at least three times. All data represent means ± SEMs. *, Compared with the control group, *p < 0.05; **p < 0.01 and ***p < 0.001. #, Comparison between intervention groups, #p < 0.05; ##p < 0.01 and ###p < 0.001