Fig. 6.

Inhibitory effect of Rg1 on M1 polarization of macrophages during MI/R injury in vitro. (A) Sytox orange dye staining of rat BMDM cells (scale bar = 10 μm); (B) Phagocytosis DNA levels in macrophages; (C–E) The M1/M2 phenotype of macrophages and the corresponding cell proportion were detected by phagocytosis flow cytometry; (F) Western blot; (G–H) The protein expression levels of iNOS and Arg-1. (I–J) The mRNA expression of iNOS and Arg-1. Results are presented as means ± SD; ^##P < 0.01 vs. Control group, ∗∗P < 0.01 vs. Nec DNA group.