Fig. 4.

GRh2 initiates cytoprotective autophagy in melanoma cells. (A) Effect of GRh2 on LC3B-II protein expression in melanoma cells. (B and C) Immunoblotting analysis of LC3B protein expression in melanoma cells following GRh2 treatment with or without (B) CQ (25 μM) and (C) Baf-A1 (25 nM) pretreatment. (D) GRh2 promotes autophagic flux in B16F10 and A375 cells. scale bar = 25 μm. (E) GRh2 lowered phospho-mTOR protein level in melanoma cells. Viabilities of GRh2-plus-CQ or -Baf-A1-treated B16F10 (F) and A375 (G) cells. ∗∗P < 0.01 vs. control group. ^#P < 0.05, ^##P < 0.01.