Fig. 5.

Oosporein biosynthetic gene cluster regulation in B. bassiana mutants with disrupted Bbsmr1 and off-target detection by whole genome sequencing analysis. A) Fold change in gene expression of Bbsmr1, OpS1-7, OpS11 and OpS12 genes in the ΔBbsmr1 mutants normalized to wild-type (WT) expression levels. Asterisks indicate significant differences between the WT and the mutants (P < 0.05). B) Read mapping of WT and in the Bbsmr1 mutant sequencing reads to the genome of ΔBbsmr1_3, confirming the disruption and location of the insertions