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. 2024 Nov 21;11:21. doi: 10.1186/s40694-024-00190-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 6 — Phenotypic characterization of the mutant strains. A) Blastospore production by knockout B. bassiana mutants (ΔBbsmr1) compared with the wild-type (WT) strain during liquid culture growth at 250 rpm and 28 °C for three days. Lettering of means (± SE) indicates statistical differences based on the Tukey’s test (P < 0.05). Symbols represent observational data in each treatment (n = 8). B. bassiana knockout mutants (ΔBbsmr1) displaying varied growth behavior when grown on potato-dextrose-agar (PDA) without the selectable marker. B) Colony growth kinetics expressed in area (cm²) for three mutants (ΔBbsmr1) and WT, and colony conidiation recorded after 12 days post-inoculation. Lettering of means (± SE, n = 6) within each time interval indicates statistical differences based on the Tukey’s test (P < 0.05). C) Morphology of fungal colonies grown on PDA after 12 days post-inoculation