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. 2024 Nov 22;24:1109. doi: 10.1186/s12870-024-05758-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 9 — Changes in DEGs and DAMs involved in the main metabolic pathways in potato stolons under different nitrogen concentrations. (A) Alpha-linolenic acid metabolism. PLA1: phospholipase A1; PLA2: phospholipase A2; LOX: lipoxygenase; HPL1: hydroperoxide lyase; AOS: allene oxide synthase; AOC: allene oxide cyclase; OPR: 12-oxophytodienoic acid reductase; OPCL: OPC-8:0 CoA ligase; ACX: acyl-CoA oxidase; KAT2: 3-ketoacyl-CoA thiolase 2; MFP2: multifunctional protein. (B) Starch and sucrose metabolism. SPS: sucrose‒phosphate synthase; SPP: sucrose‒phosphatase; SUS: sucrose synthase; ENPP: ectonucleotide pyrophosphatase; EG: endoglucanase; AGPS: glucose‒1‒phosphate adenylyltransferase; SS: starch synthase; BE: 1,4‒glucan branching enzyme; AMY: alpha‒amylase; BMY: beta‒amylase. (C) Nitrogen metabolism. NR: nitrate reductase; NiR: ferredoxin-nitrite reductase; Nrt: nitrite transporter; GS: glutamine synthetase; GOGAT: glutamate synthase; GDH: glutamate dehydrogenase; CA: carbonic anhydrase; and CYN: cyanate hydratase. Red represents relatively highly expressed genes and metabolites, blue represents relatively poorly expressed genes and metabolites, and genes that met the P < 0.05 and |log₂-fold changes|>1 thresholds were defined as DEGs. Metabolites that met the P < 0.05 and VIP > 1 thresholds were defined as DAMs