Table 1.
Primer sequences (sense (s) and anti-sense (as)), melting temperatures (Tm), annealing temperature and expected product size in nucleotides (nt) for each of the seven selected marker genes.
| Name | Sequence 5' – 3' | Tm | Annealing | Size |
|---|---|---|---|---|
| MUC1 s | ACCAAGACTGATGCCAGTAGCACT | 65°C | ||
| MUC1 as | ACCGTTACCTGCAGAAACCTTCT | 63°C | 68°C | 115 nt |
| ESX s | CTCGGAGCTCCCACTCCTCAGA | 68°C | ||
| ESX as | GCTCTTCTTGCCCTCGAGACAGT | 67°C | 68°C | 188 nt |
| CEA s | GGTTGGGGTTGCTCTGATATAGCAGC | 70°C | ||
| CEA as | GCTGTTGCAAATGCTTTAAGGAAGAAGC | 67°C | 66°C | 97 nt |
| EPHB4 s | CCCCAGGGAAGAAGGAGAGCTG | 68°C | ||
| EPHB4 as | GCCCACGAGCTGGATGACTGTG | 68°C | 68°C | 250 nt |
| EGFR s | TGTGAGGTGGTCCTTGGGAATTTGG | 67°C | ||
| EGFR as | TGCTGACTATGTCCCGCCACTGGA | 69°C | 66°C | 339 nt |
| EpCAM s | GGACCTGACAGTAAATGGGGAAC | 65°C | ||
| EpCAM as | CTCTTCTTTCTGGAAATAACCAGCAC | 65°C | 68°C | 186 nt |
| MGB1 s | CGGATGAAACTCTGAGCAATGTTGAG | 66°C | ||
| MGB1 as | CTGCAGTTCTGTGAGCCAAAGGTC | 67°C | 68°C | 110 nt |
Primer sequences (sense (s) and anti-sense (as)), melting temperatures (Tm), annealing temperature and expected product size in nucleotides (nt) for each of the seven selected marker genes. The TM (Salt adjusted) was calculated using the web-based Oligonucleotide Properties calculators http://www.basic.nwu.edu/biotools/oligocalc.html.