Table 2.
Comparison of the expression of the seven selected markers in either 100 PBMNCs only, or after the addition of a single tumor cell from one of four different cell lines.
| ELF3 | EpCam | EphB4 | MUC1 | CEA | EGFR | MGB1 | |
|---|---|---|---|---|---|---|---|
| Cycles | 45 | 45 | 45 | 45 | 55 | 45 | 45 |
| 100 PBMNC | 5/30 | 0/21 | 0/21 | 1/6 | 0/18 | 0/27 | 0/24 |
| 453 | 17/18 | 5/5 | 9/10 | 5/10 | 0/10 | 8/18 | 4/10 |
| 468 | 5/5 | 5/5 | 5/5 | ND | ND | 5/5 | ND |
| MCF-7 | 10/10 | 8/10 | 6/10 | 5/5 | 9/10 | 1/5 | 1/10 |
| T-47D | 8/10 | 13/15 | 11/15 | ND | ND | 5/15 | 4/15 |
| TOTAL | 40/43 | 31/35 | 31/40 | 10/15 | 9/20 | 19/43 | 9/35 |
Comparison of the expression of the seven selected markers in either 100 PBMNCs only, or after the addition of a single tumor cell from one of four different cell lines (MDA-MB-453, MDA-MB-468, MCF-7 and T47-D) after 45 or 55 rounds (CEA) of PCR amplification. For example EphB4 was detected in 9 out of 10 single MDA-MB-453 cells added to 100 PBMNC aliquots. ND- test not performed.