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. 2002 May 24;2:14. doi: 10.1186/1471-2407-2-14

Table 3.

Profile of gene expression from 10 different experiments each using 5 single tumour cell/100 PBMNC aliquots from four different cell lines.

MDA-MB-468 MDA-MB-453 MDA-MB-453 MDA-MB-453

1 2 3 4 5 1 2 3 4 5 1 2 3 4 5 1 2 3 4 5
ELF3 + + + + + + + + + + + + + + + + + + + +
EphB4 + + + + + + + + + + + + + + + + + + + -
EpCam + + + + + + + + + + + + + + +
EGFR + + + + + + - - + + - + - + - + + - - -
MGB1 - - - - - - - - + - + - - + +
T-47D T-47D T-47D T-47D

1 2 3 4 5 1 2 3 4 5 1 2 3 4 5 1 2 3 4 5

ELF3 + + + + + + + + + + + + - + -
EphB4 + + + + + - + + - + + + + + + + + - + -
EpCam + + + + + + + + + + + - + + + + + + + -
EGFR - - - - - - + - - - - - - - + + + + - -
MGB1 - - - - - - - - + + - - - - - + + - - -
MCF-7 MCF-7

1 2 3 4 5 1 2 3 4 5

ELF3 + + + + + + + + + +
EphB4 - + + + + - + - + -
EpCam - + + - + + + - + +
EGFR - + - - - + - - - -
MGB1 - - - - - - - - - -

Profile of gene expression from 10 different experiments each using 5 single tumour cell/100 PBMNC aliquots from four different cell lines demonstrating heterogeneity of gene expression and utility of several markers for accurate detection of the single tumour cell. A "+" indicates that marker was detected, a "-" indicates that marker was not detected, and a blank indicates that marker was not done.